Oxion fluorescence- OX.3085

Euomex, OX.3237, Trinocular, Plan semi apo fluarex 4x/10x/S40x objectives, Plan semi apo fluarex S100x objectives

For biological and medical applications fluorescence microscopes are used to observe certain parts of living cells and tissues with help of fluorophores. One or more fluophores are added to the parts of the specimen to be observed. When the fluorophores are exposed to so-called exicitation light, the excitation energy is absorbed by the fluorophore that start emitting so-called emission light that can be observed by the microscope. One can distinguish tumor cells from other cells, follow biological processes or prove the presence or absence of antibodies, etc.

SKU: OX.3085 Categories: , Tags: , , , ,

Description

For biological and medical applications fluorescence microscopes are used to observe certain parts of living cells and tissues with help of fluorophores. One or more fluophores are added to the parts of the specimen to be observed. When the fluorophores are exposed to so-called exicitation light, the excitation energy is absorbed by the fluorophore that start emitting so-called emission light that can be observed by the microscope. One can distinguish tumor cells from other cells, follow biological processes or prove the presence or absence of antibodies, etc

The Oxion microscopes for fluorescence are equipped with an epi-illumination with a 100 W mercury vapor lamp. The fluorescence attachment have a rail for maximum 4 filter cubes. The FluoLed model is equipped with a 455 nm LED and one filter cube, especially developed for efficient and fast detection of tuberculosis by the Auramine-O fluorochrome. Other FluoLED wavelengths are also available

Highlights

  • Binocular and trinocular models
  • Plan, plan fluarex and plan semi apochromatic fluarex ioS objectives
  • Coaxial coarse and fine adjustments
  • 3 W lED transmitted illumination
  • Fluorescence attachment with single filter block and 455 nm (Blue), 560 nm (Green) or 365 nm (UV) in
  • HWF 10x/22 mm eyepieces
  • Reversed nosepiece for maximum 5 objectives
  • Integrated mechanical stage
  • Height adjustable abbe n.a. 1.25 condenser with iris diaphragm and filter holder
  • Fluorescence attachment for maximum 4 filter blocks and with 100 W mercury vapor incident illuminati
  • Ergonomic stand and head

  • Specifications:
  • EYEPIECE(S)
    HWF 10x/22 mm eyepieces. All eyepieces can be secured by an Allen screw

    HEADS
    Binocular and trinocular Siedentopf type heads are with 30° inclined tubes. Interpupillary distance from 50 to 75 mm. Two ± 5 diopter adjustments. A unique rotating system allows the ergonomical positioning of both tubes in a high (431 mm) and in a low position (397 mm)

    NOSEPIECE
    Revolving and reversed nosepiece for maximum 5 objectives. The fluorescence models with mercury vapor lamp are equipped with an orange UV protection shield

    OBJECTIVES
    • Plan 4x/0.10, 10x/0.25, S40x/0.65 and S100x/1.25 oil immersion IOS objectives
    • Plan fluarex 4x/0.10, 10x/0.25, S40x/0.65 and S100x/1.25 oil immersion IOS objectives
    • Plan semi apochromatic fluarex 4x/0.10, 10x/0.25, S40x/0.65 and S100x/1.25 oil immersion IOS objectives

    All S40x, S60x and S100x oil immersion objectives are spring loaded. All optics are anti-fungus treated and anti-reflection coated for maximum light throughput

    FOCUSING
    Coaxial coarse and fine adjustments, 200 graduations,1 μm per graduation, 200 μm per rotation, total travel approximately 28 mm. Supplied with an adjustable rack stop to prevent damage to sample and objectives. The coarse adjustments are equipped with friction control

    STAGE
    150 x 140 mm stage with integrated X-Y mechanical stage. A ceramic stage is also available on request

    CONDENSER FOR BRIGHTFIELD
    Height adjustable Abbe condenser N.A. 1.25 with iris diaphragm. Supplied with a white diffusion filter. Phase contrast rings mounted in slider can be inserted in the condenser for 10x and S40x or 20x and S100x phase contrast objectives. A dark field stop in slider for the 10x, 20x and S40x objectives can also be inserted with magnification indication

    CONDENSER FOR FLUORESCENCE
    The fluorescence attachment is equipped with an integrated condenser with iris diaphragm and with a field diaphragm

    ILLUMINATION FOR BRIGHTFIELD AND PHASE CONTRAST
    All models are supplied with a 3 W adjustable NeoLED illumination with built-in 100-240 V power supply. Köhler illumination with field diaphragm

    ILLUMINATION FOR FLUORESCENCE
    Fluorescence attachment with 100 W (HBO) mercury vapor incident epi-illumination and rail for maximum 4 filter blocks. Two general purpose filter sets are standard supplied with the attachment: AE.3245 (Blue excitation) and AE.3246 (Green excitation). Two other general purpose filter sets AE.3246 (UV excitation) and AE.3247 (Ultraviolet excitation) are also available. More specific filter sets are available on request

    STANDARD GENERAL PURPOSE FLUORESCENCE FILTER SETS

    OXION_fluor_graphics

    ILLUMINATION FOR FLUOLED ATTACHMENT
    The FluoLed attachment is standard delivered with a 455 nm LED for efficient and fast detection of tuberculosis with Auramine-O fluorochrome. FluoLED attachments are also available with a 560 nm LED and Green filter set or a 365 nm LED together with an Ultraviolet filter set

    CONFIGURATIONS
    The Oxion microscope is available in many configurations and can be upgraded with a variety of contrast types.

    standard models
    • phase contrast models

    dark field models
    • inverso (life science) models
    material science models
    inverso (material science) models
    • fluorescence models

    PACKAGING
    Supplied with power cord, dust cover, white filter, user manual and 5 ml immersion oil for models with S100x objective. All packed in a polystyrene box.

    Accessories:
    AE.3210 HWF 10x/22 mm eyepiece
    AE.3215 HWF 15x/13 mm eyepiece
    AE.3223 HWF 10w/22mm eyepiece with 10mm/100 micrometer reticule
    AE.3225 Pair of eyecups
    AE.3160 Infinity semi plan SMP 4x/0.10 IOS objective. Working distance 21 mm
    AE.3162 Infinity semi plan SMP 10x/0.25 IOS objective . Working distance 5,95 mm
    AE.3166 Infinity semi plan SMP S40x/0.65 IOS objective. Working distance 0,64 mm
    AE.3270 Infinity semi plan SMP S100x/1.25 oil immersion IOS objective. Working distance 0,36 mm
    AE.3102 Infinity plan PL 2x/0,06 IOS objective
    AE.3104 Infinity plan PL 4x/0.10 IOS objective. Working distance 11,9 mm
    AE.3106 Infinity plan PL 10x/0.25 IOS objective. Working distance 12,1 mm
    AE.3108 Infinity plan PL 20x/0.40 IOS objective. Working distance 1,5 mm
    AE.3110 Infinity plan PL S40x/0.65 IOS objective. Working distance 0,36 mm
    AE.3112 Infinity plan PL S60x/0.85 IOS objective. Working distance 0,3 mm
    AE.3114 Infinity plan PL S100x/1.25 oil immersion IOS objective. Working distance 0,18 mm
    86.550 Plan Fluarex PLF 4x /0,15 infinity corrected IOS objective. Working distance 21,6 mm
    AE.3144 Plan semi apochromatic Fluarex PLF 4x/0,13 infinity corrected IOS objective
    AE.3146 Plan semi apochromatic Fluarex PLF 10x/0,30 infinity corrected IOS objective
    AE.3148 Plan semi apochromatic Fluarex PLF 20x/0,50 infinity corrected IOS objective
    AE.3152 Plan semi apochromatic Fluarex PLF S40x/0,75 infinity corrected IOS objective
    AE.3156 Plan semi apochromatic Fluarex PLF S100x /1,28 oil immersion infinity corrected IOS objective Working distance 0,21 mm
    AE.3240 Fluorescence illuminator with 100 Watt mercury vapor lamp, inclusive power supply unit and UV protection shield
    AE.3245 Fluorescence block with general purpose filter set for Blue EX475BP40, DM505LP,EM535BP45. Excellent for FITC, eGFP. Compatible for 5-carboxyfluorescein (5-FAM), Alexa fluor 488, BODIP-FL, Calcein, Cy2, Dio (Dio 18(3)), eGFP, FITC, Fluo-4, Fluor-Jade, FluorX, iLyte Fluor 488, Mito tracker Green, Rhodamine 110, Spectrumgreen 110, SYTO-13, YO-PRO-1, YOYO-1
    AE.3246 Fluorescence block with general purpose filter set for Green EM560BP55, DM595LP, EM645BP75. Excellent for Texas Red, Alexa 594, 5-Carboxynaphthofluorescein. Other compatible fluorophores: 5-CNF, 5-ROX (carboxy-X-rhodamine),Alexa Fluor 568, Alexa Fluor 594, BOBO-3, BODIPY-TR-X, BO-PRO-3, Calcium Crimson, Cy3.5, HcRed, HiLyte Fluor 594, Lissamine rhodamine B, Mito Tracker Red, mPlum mRaspberry, resorufin, Rhodamine Red, R-phycocyanin, SpectrumRed, Texas Red, X-Rhodaline (XRITC), YO-PRO-3, YOYO-3
    AE.3247 Fluorescence block with general purpose filter set for Violet EX440BP21, DM455LP, EM480BP30. Compatible fluorophores: BOBO-1, BOPRO-1, Cerulean, CFP, POPO-1, PO-PRO-1, SpectrumAqua, SYTO45, SYTOX Blue
    AE.3248 Fluorescence block with general purpose filter set for UV EX365BP50, DM400LP, EM450BP40. Compatible fluorophores: 4-Methylumbelliverone, 7-amino-4methylcoumarin, 7-Hydrixy-4-methylcoumarin (pH9), Alexa Fluor 350, AMCA, AMCA-X, Calcein Blue, Calcein White, Cascade Blue, DAPI, Hoechst 33342 & 33258, LysoSensor Blue (pH5), Lyso Tracker Red, marina Blue, Spectrum Blue
    OX.9750.0401 Premium fluorescence filter set EX440BP21 DM455LP EM480BP30 optimized for CFP. Compatible with eCFP, mCFPm, Cerulean, CyPet adn SpectrumAqua. This filter set is designed for optimal signal capture of CFP and to minimize spectral bleed-through of YFP. Needs empty block OX.9749
    OX.9750.0403 Premium fluorescence filter set EX380BP50 DM410LP EM450BP60 optimized for BFP, BFP2. Compatible with AlexaFluor 350, DAPI; Hoeschst 33342 & 33258. Wide excitation filter bandwidth may cause cellular damage in live cell applications. Needs empty block OX.9749
    OX.9750.0404 Premium fluorescence filter set EX470BP40 DM500LP EM535BP50 optimized for AlexaFluor 488. Compatible with Cy2, eCFP, FITC and GPT (sapphire). Designed for both excellent brightness and contrast with greater than OD 6 at the excitation/emission crossover. Needs empty block OX.9749
    OX.9750.0407 Premium fluorescence filter set EX635BP30 DM660LP EM710BP80 optimized for AlexaFluor 647. Compatible with Cy5, TOTO-1, TOTO-3, Kodak X-Sight 650. A wide emission filter for maximum photon capture. A narrow excitation filter. Needs empty block OX.9749
    OX.9750.0412 Premium fluorescence filter set EX500BP25 DM525LP EM545BP35 optimized for YFP and for minimizing CFP bleedthrough. Compatible with AlexFluor 532, Acridine Orange (+DNA), Rhodamine Green . Needs empty block OX.9749
    OX.9750.0414 Premium fluorescence filter set EX560BP55 DM595LP EM645BP75 optimized for Texas Red. Compatible with Texas Red-X, Mito Tracker Red, Cy 3,5, HcRed, mRasberry. Wide passbands that give high brightness and contrast to red emitting fluorophores such as Texas Red. Needs empty block OX.9749
    OX.9750.39001 Fluorescence filter set EX435 (BP20) DM455 (LP) EM480 (BP30) optimzed for AmCyan, Atto 425, Cerulean, CFP, ECFP fluorophore. Needs empty filter block (OX.9749)
    OX.9750.39002 Fluorescence filter set EX480 (BP30) DM505(LP) EM535 (BP40) optimized for Acridine Orange + DNA, Alexa Fluor 488™, Atto 488, Azami Green, BODIPY FL/pH7.2, Calcein, Calcium, Green™-1, Cy2™, DiO, DyLight 488, EGFP, Emerald GFP, FAM, FITC, Fluo-4, GFP, LysoTracker Green/pH 5.2, MitoTracker Green, FM/MeOH, mWasabi, Oregon Green™ 488, SYBR® Green I, SYTO 9/DNA, ZsGreen fluorophore. Needs empty filter block (OX.9749)
    OX.9750.39003 Fluorescence filter set EX495 (BP20) DM515 (LP) EM540 (BP30) optimized for Alexa Fluor 514™, Citrine, EYFP/pH 7, F/lAsH-CCPFCC, mCitrine, Oregon Green™ 514, Rhodamine 123, Topaz. Needs empty filter block (OX.9749)
    OX.9750.39004 Fluorescence filter set EX540 (BP25) DM565 (LP) EM605 (BP55) optimized for Alexa Fluor 546™, Alexa Fluor 555™, Atto 550, CAL, Fluor® Red 590, Cy3™, DiI, DsRed, DyLight 549, MitoTracker Orange/MeOH, Resorufin, Rhod-2, TagRFP, TAMRA tdTomato, Tetramethylrhodamine, isothiocyanate, TRITC. Needs empty filter block (OX.9749)
    OX.9750.39005 Fluorescence filter set EX540 (BP25) DM565 (LP) EM 620 (BP60) optimized TRITC-red-shifted, Ethidium homidimer-1/DNA, Propidium Iodide. Needs empty filter block (OX.9749)
    OX.9750.39007 Fluorescence filter set EX620 (BP50) DM655 (LP) EM 690 (BP50) optimized for Alexa Fluor 647™, Allophycocyanin (APC), Atto 647N, Cy5™, DiD, Draq5, DyLight 649 MitoTracker Deep Red 633/MeOH, Nile Blue, Quasar® 670, SYTO® 60, TO-PRO™-3. Needs empty filter block (OX.9749)
    OX.9750.39008 Fluorescence filter set EX420 (BP40) DM455 (LP) EM 535 (BP40) optimized for Lucifer Yellow/Tetracycline/AlexaFluor 430. Needs empty filter block (OX.9749)
    OX.9750.39009 Fluorescence filter set EX480 (BP30) DM505 (LP) EM 620 (BP60) for Acridine Orange + RNA, Di-8-ANEPPS, non-ratiometric, DiA, Ethidium Bromide, FM™ 1-43, LysoTracker Yellow HCK-123, Pulsar™ 650. Needs empty filter block (OX.9749)
    AE.5120 Standard 23,2 mm tube for Oxion upright (revision 2) and Oxion Inverso inverted microscopes
    AE.5130 Universal Ø 23.2 mm tube adapter with built-in 2x lens for SLR photo camera with APS-C sensor. Needs T2 adapter
    AE.5025 T2 ring for Nikon D SLR digital camera
    AE.5040 T2 ring for Canon EOS SLR digital camera
    AE.5057 C-mount adapter 0,5x for 1/2 inch cameras for Oxion (revision 1)
    AE.5058 C-mount adapter 0,33x adapter for 1/3 inch camera for Oxion (revision 1)
    OX.9833 Photo port adapter with 0,33x lens for Oxion (revision 2) and Oxion Inverso microscopes and 1/3 inch camera with C-mount
    OX.9850 Photo port adapter with 0,5x lens for Oxion (revision 2) and Oxion Inverso microscopes and 1/2 inch camera with C-mount
    AE.1385 100 W HBO mercury lamp for microscopes with fluorescence attachment
    AE.3199 Glass fuses 3.15A 250V, 10pcs
    SL.5511 3W NeoLED™ replacement unit for Oxion
    PB.5155 Microscope slides 76 x 26 mm, ground edges, 50 pieces
    PB.5165 Cover glasses 18 x 18 mm, 0,13-0,17 mm, 100 pieces
    PB.5255 Immersion oil, 25 ml. Refraction index n = 1,482
    PB.5274 Iso propyl alcohol 99%, 200 ml
    PB.5275 Cleaning kit: lens fluid, lint free lens tissue/paper, brush, air blower, cotton swabs

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